Cell Culture:Article Title: CRISPR/Cas9-mediated editing of VHL in induced pluripotent stem cells: A model for early cell fate in von Hippel-Lindau syndrome.
Article Snippet: .. Unique stem cell lines identifier IUFi004-A-10 IUFi004-A-11 (please see cover letter about the submission of the lines) Alternative name(s) of stem cell lines DU402 DU407 Institution IUF Leibniz research institute for Environmental Medicine Contact information of distributor Andrea Rossi; andrea.rossi@iuf-duesseldorf.de Type of cell lines iPSC Origin Human Additional origin info required for human ESC or iPSC Age: 64 Sex: female Cell Source iPS12-10 (IUFi004-A), Cell Applications Inc, https://www.cellapplications. com/human-induced-pluripotent-stem-cells-hipsc Method of reprogramming N/A Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A The cell culture system used Feeder cell free cell culture, iMatrix (continued on next column) (continued ) Type of Genetic Modification Induced Mutation (out-of-frame indels) Associated disease Von Hippel-Lindau syndrome (VHL) Gene/locus IUFi004-A-10: VHL homozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.331_338del, c.333del IUFi004-A-11: VHL heterozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.337_365del) Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method PX458 (Cas9), plasmid, LipofectamineTMStem All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (PX458) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. ..
Modification:Article Title: CRISPR/Cas9-mediated editing of VHL in induced pluripotent stem cells: A model for early cell fate in von Hippel-Lindau syndrome.
Article Snippet: .. Unique stem cell lines identifier IUFi004-A-10 IUFi004-A-11 (please see cover letter about the submission of the lines) Alternative name(s) of stem cell lines DU402 DU407 Institution IUF Leibniz research institute for Environmental Medicine Contact information of distributor Andrea Rossi; andrea.rossi@iuf-duesseldorf.de Type of cell lines iPSC Origin Human Additional origin info required for human ESC or iPSC Age: 64 Sex: female Cell Source iPS12-10 (IUFi004-A), Cell Applications Inc, https://www.cellapplications. com/human-induced-pluripotent-stem-cells-hipsc Method of reprogramming N/A Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A The cell culture system used Feeder cell free cell culture, iMatrix (continued on next column) (continued ) Type of Genetic Modification Induced Mutation (out-of-frame indels) Associated disease Von Hippel-Lindau syndrome (VHL) Gene/locus IUFi004-A-10: VHL homozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.331_338del, c.333del IUFi004-A-11: VHL heterozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.337_365del) Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method PX458 (Cas9), plasmid, LipofectamineTMStem All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (PX458) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. ..
Mutagenesis:Article Title: CRISPR/Cas9-mediated editing of VHL in induced pluripotent stem cells: A model for early cell fate in von Hippel-Lindau syndrome.
Article Snippet: .. Unique stem cell lines identifier IUFi004-A-10 IUFi004-A-11 (please see cover letter about the submission of the lines) Alternative name(s) of stem cell lines DU402 DU407 Institution IUF Leibniz research institute for Environmental Medicine Contact information of distributor Andrea Rossi; andrea.rossi@iuf-duesseldorf.de Type of cell lines iPSC Origin Human Additional origin info required for human ESC or iPSC Age: 64 Sex: female Cell Source iPS12-10 (IUFi004-A), Cell Applications Inc, https://www.cellapplications. com/human-induced-pluripotent-stem-cells-hipsc Method of reprogramming N/A Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A The cell culture system used Feeder cell free cell culture, iMatrix (continued on next column) (continued ) Type of Genetic Modification Induced Mutation (out-of-frame indels) Associated disease Von Hippel-Lindau syndrome (VHL) Gene/locus IUFi004-A-10: VHL homozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.331_338del, c.333del IUFi004-A-11: VHL heterozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.337_365del) Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method PX458 (Cas9), plasmid, LipofectamineTMStem All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (PX458) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. ..
Article Title: Generation of an iPSC line IUFi004-A-13 with homozygous NDUFS1 mutation for the study of Leigh syndrome.
Article Snippet: .. Type of cell line Induced pluripotent stem cells (iPSC) Origin Human Additional origin info (applicable for human ESC or iPSC) Age: 64 Sex: female Cell Source iPS12-10 (IUFi004-A), Cell Applications Inc, https://www.cellapplications.com (continued on next column) (continued ) /human-induced-pluripotent-stem-cells-h ipsc Method of reprogramming NA Clonality The desired mutation (c.691C > G) is present in both alleles (homozygous). ..
CRISPR:Article Title: CRISPR/Cas9-mediated editing of VHL in induced pluripotent stem cells: A model for early cell fate in von Hippel-Lindau syndrome.
Article Snippet: .. Unique stem cell lines identifier IUFi004-A-10 IUFi004-A-11 (please see cover letter about the submission of the lines) Alternative name(s) of stem cell lines DU402 DU407 Institution IUF Leibniz research institute for Environmental Medicine Contact information of distributor Andrea Rossi; andrea.rossi@iuf-duesseldorf.de Type of cell lines iPSC Origin Human Additional origin info required for human ESC or iPSC Age: 64 Sex: female Cell Source iPS12-10 (IUFi004-A), Cell Applications Inc, https://www.cellapplications. com/human-induced-pluripotent-stem-cells-hipsc Method of reprogramming N/A Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A The cell culture system used Feeder cell free cell culture, iMatrix (continued on next column) (continued ) Type of Genetic Modification Induced Mutation (out-of-frame indels) Associated disease Von Hippel-Lindau syndrome (VHL) Gene/locus IUFi004-A-10: VHL homozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.331_338del, c.333del IUFi004-A-11: VHL heterozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.337_365del) Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method PX458 (Cas9), plasmid, LipofectamineTMStem All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (PX458) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. ..
Plasmid Preparation:Article Title: CRISPR/Cas9-mediated editing of VHL in induced pluripotent stem cells: A model for early cell fate in von Hippel-Lindau syndrome.
Article Snippet: .. Unique stem cell lines identifier IUFi004-A-10 IUFi004-A-11 (please see cover letter about the submission of the lines) Alternative name(s) of stem cell lines DU402 DU407 Institution IUF Leibniz research institute for Environmental Medicine Contact information of distributor Andrea Rossi; andrea.rossi@iuf-duesseldorf.de Type of cell lines iPSC Origin Human Additional origin info required for human ESC or iPSC Age: 64 Sex: female Cell Source iPS12-10 (IUFi004-A), Cell Applications Inc, https://www.cellapplications. com/human-induced-pluripotent-stem-cells-hipsc Method of reprogramming N/A Clonality Clonal Evidence of the reprogramming transgene loss (including genomic copy if applicable) N/A The cell culture system used Feeder cell free cell culture, iMatrix (continued on next column) (continued ) Type of Genetic Modification Induced Mutation (out-of-frame indels) Associated disease Von Hippel-Lindau syndrome (VHL) Gene/locus IUFi004-A-10: VHL homozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.331_338del, c.333del IUFi004-A-11: VHL heterozygous 3p25-26 Exon 1 deletion (NM_000551.4: c.337_365del) Method of modification / user-customisable nucleases (UCN) used, the resource used for design optimisation CRISPR/Cas9 User-customisable nuclease (UCN) delivery method PX458 (Cas9), plasmid, LipofectamineTMStem All double-stranded DNA genetic material molecules introduced into the cells pSpCas9(BB)-2A-GFP (PX458) Evidence of the absence of random integration of any plasmids or DS DNA introduced into the cells. ..
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